9CEA

Crystal structure of HP1alpha chromoshadow domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-24
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	63.758, 66.854, 70.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.180 - 2.150
R-factor	0.22982
Rwork	0.227
R-free	0.27732
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.924
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.180	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.129	0.916
Rmeas	0.140	1.000
Rpim	0.055	0.400
Number of reflections	17068	1446
<I/σ(I)>	11.3
Completeness [%]	99.9
Redundancy	6.4	6.2
CC(1/2)	0.998	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M HEPES pH 7, 0.2 M sodium chloride and 20% w/v PEG6000