Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.429, 39.775, 62.471 |
| Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
| Resolution | 47.730 - 1.500 |
| Rwork | 0.206 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PRIME-X |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.730 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 1.336 |
| Rmeas | 0.075 | |
| Rpim | 0.049 | |
| Number of reflections | 43736 | 2168 |
| <I/σ(I)> | 12.2 | 0.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.1 | 3.8 |
| CC(1/2) | 0.999 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15.9% PEG 3350, 0.1M Magnesium Acetate, 0.1M HEPES |
