9CA6
Rns Pocket Mutant - I14F/I17F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979351 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.810, 96.680, 138.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.200 - 3.000 |
| R-factor | 0.3538 |
| Rwork | 0.352 |
| R-free | 0.36920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.101 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 3.100 |
| High resolution limit [Å] | 2.995 | 3.000 |
| Number of reflections | 24421 | 2403 |
| <I/σ(I)> | 2.32 | |
| Completeness [%] | 99.0 | 98.91 |
| Redundancy | 2.8 | |
| CC(1/2) | 0.928 | 0.146 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1:1 v/v DMSO, 0.1 M succinnic acid, 14% PEG 3350, 0.03 M glycyl-glycyl-gylcine |
