9C97
Yeast 20S proteasome soaked with BRA-346 fraction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9772 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.511, 299.917, 143.913 |
| Unit cell angles | 90.00, 108.46, 90.00 |
Refinement procedure
| Resolution | 49.110 - 3.330 |
| R-factor | 0.2028 |
| Rwork | 0.200 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 3.390 |
| High resolution limit [Å] | 3.330 | 3.330 |
| Rmerge | 0.395 | 0.793 |
| Rmeas | 0.483 | 0.973 |
| Rpim | 0.274 | 0.556 |
| Number of reflections | 132592 | 6605 |
| <I/σ(I)> | 3 | 1.1 |
| Completeness [%] | 96.5 | 97.4 |
| Redundancy | 3 | 2.9 |
| CC(1/2) | 0.754 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | MES 100 mM pH 6.9, MPD 25% and magnesium acetate 20 mM |
