9C95
Apo crystal structure of AprG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.160, 76.820, 123.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.450 - 1.420 |
| R-factor | 0.1645 |
| Rwork | 0.164 |
| R-free | 0.18120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.073 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18rc3_3805) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.079 | 1.192 |
| Rmeas | 0.087 | 1.334 |
| Rpim | 0.035 | 0.588 |
| Total number of observations | 760051 | 30799 |
| Number of reflections | 124336 | 6154 |
| <I/σ(I)> | 12 | 1.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.1 | 5 |
| CC(1/2) | 0.998 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.2M Calcium Acetate, 0.1M Sodium Cacodylate pH 6.0, 18% PEG 8000 |
