9C8Z

X-ray crystal structure of Methylorubrum extorquens Eu(III)-bound LanD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92011
Spacegroup name	I 21 3
Unit cell lengths	78.334, 78.334, 78.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.980 - 1.410
R-factor	0.1976
Rwork	0.197
R-free	0.20470
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.957
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.980	1.460
High resolution limit [Å]	1.410	1.410
Number of reflections	14522	575
<I/σ(I)>	24.7
Completeness [%]	93.3
Redundancy	7.6
CC(1/2)	0.997	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM MES, pH 6.5, 25% w/v PEG200