9C8W

X-ray crystal structure of Methylorubrum extorquens apo LanD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	76.776, 23.507, 76.446
Unit cell angles	90.00, 120.02, 90.00

Refinement procedure

Resolution	38.390 - 1.650
R-factor	0.2141
Rwork	0.212
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.816
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.390	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	21758	1073
<I/σ(I)>	28.4
Completeness [%]	96.5
Redundancy	7.2
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M potassium fluoride, 20% w/v PEG3350