9C7Z

Hallucinated C3 protein assembly HALC3_919

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 3
Unit cell lengths	78.373, 78.373, 78.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.420 - 2.100
R-factor	0.2337
Rwork	0.233
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.114
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.420	2.160
High resolution limit [Å]	1.950	2.100
Rmerge	0.059	0.988
Number of reflections	9601	1344
<I/σ(I)>	15.5	2.1
Completeness [%]	99.9	99.41
Redundancy	8.8
CC(1/2)	0.999	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	291	0.2 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 4.2, 20% w/v PEG 1000