9C7N

Crystal Structure of the GL3 ACT-like Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-27
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 2 21 21
Unit cell lengths	66.859, 83.524, 88.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.762 - 2.850
R-factor	0.2421
Rwork	0.237
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.714
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.850	7.730	2.850
Rmerge	0.092	0.044	0.795
Rmeas	0.102	0.050	0.878
Rpim		0.022	0.367
Number of reflections	12015	653	612
<I/σ(I)>	6.7
Completeness [%]	99.3	95.2	100
Redundancy	5.5	4.8	5.6
CC(1/2)		0.997	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	150 mM NaCl, 10 mM Tris.HCl, 0.5 mM TCEP. HCl, pH:8.0