9C75
The crystal structure of HIV-1 Rev Response Element Stem-Loop II G34U mutant in complex with a Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97936 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 90.878, 105.014, 186.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.640 - 3.040 |
| R-factor | 0.2079 |
| Rwork | 0.205 |
| R-free | 0.25160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.226 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.640 | 3.095 |
| High resolution limit [Å] | 3.040 | 3.042 |
| Rmerge | 0.183 | 2.518 |
| Rmeas | 0.198 | 2.723 |
| Rpim | 0.076 | 1.028 |
| Number of reflections | 17512 | 855 |
| <I/σ(I)> | 7.1 | 0.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.997 | 0.383 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2M NaCl, 0.1M Tris pH 8.0, 12.5% polyvinylpyrrolidone, 10% PEG 4000 |
