9C73
Hybrid G-quadruplex from Tetrahymena thermophila telomeric sequence in complex with TrisQO
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 196 |
| Detector technology | PIXEL |
| Collection date | 2023-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 54.913, 54.913, 98.701 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.460 - 1.660 |
| R-factor | 0.2149 |
| Rwork | 0.214 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.097 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX (1.19.2_4158) |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.556 | 1.684 |
| High resolution limit [Å] | 1.656 | 1.656 |
| Rmerge | 4.554 | |
| Number of reflections | 11080 | 528 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.6 | 15.2 |
| CC(1/2) | 0.999 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M potassium chloride, 0.01 calcium chloride dihydrate, 0.05 M HEPES sodium pH 7.0, 10% v/v PEG 400 |
