9C72

Structure of the ASH3 domain of Drosophila melanogaster Spd-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	I 41 2 2
Unit cell lengths	88.580, 88.580, 79.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.640 - 1.930
R-factor	0.1747
Rwork	0.173
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.046
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.640	2.120
High resolution limit [Å]	1.930	1.930
Number of reflections	12013	2936
<I/σ(I)>	15.1
Completeness [%]	99.1
Redundancy	5.6
CC(1/2)	0.998	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2M ammonium sulfate, 5% PEG400, 0.1M HEPES