9C6A

The CRISPR associated adenosine deaminase Cad1-CARF in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	53.615, 96.801, 155.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.210 - 3.600
R-factor	0.2521
Rwork	0.248
R-free	0.31700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.617
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP (CCP4i2)
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.210	3.930
High resolution limit [Å]	3.590	3.590
Rmerge	0.240	0.638
Rmeas	0.340	0.902
Rpim	0.240	0.638
Total number of observations	18293	4365
Number of reflections	10011	2326
<I/σ(I)>	2	1.1
Completeness [%]	100.0
Redundancy	1.8	1.9
CC(1/2)	0.923	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
apoCad1CARF	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris-HCl pH 8.5, 2 M ammonium sulfate