9C69

The CRISPR associated CARF-adenosine deaminase, Cad1-CARF in the cA4 bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	293
Detector technology	PIXEL
Collection date	2024-02-04
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9793
Spacegroup name	I 4 3 2
Unit cell lengths	189.308, 189.308, 189.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.330 - 2.400
R-factor	0.2041
Rwork	0.202
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.065
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.330	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.099	0.537
Number of reflections	22654	2367
<I/σ(I)>	40.5
Completeness [%]	99.0
Redundancy	1	1
CC(1/2)	1.000	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
Cad1CARF-cA4	VAPOR DIFFUSION, HANGING DROP	5	293	1 M lithium chloride, 0.1 M citric acid pH 5, 10 % (w/v) PEG 6000