Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1. |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.610, 67.880, 40.170 |
| Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
| Resolution | 16.840 - 1.150 |
| R-factor | 0.1559 |
| Rwork | 0.155 |
| R-free | 0.17540 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.884 | 1.154 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.037 | 0.529 |
| Rmeas | 0.044 | 0.633 |
| Rpim | 0.024 | 0.343 |
| Number of reflections | 72070 | 2271 |
| <I/σ(I)> | 17.9 | 2.1 |
| Completeness [%] | 95.8 | 94.4 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 25% Peg 3350, 200 mM MgCl2, 100 mM Tris-Cl pH=8.5, 1 mM TCEP |
