9C5G

Structure of R. leguminosarum CapW bound to single-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	77.955, 62.008, 82.335
Unit cell angles	90.00, 93.48, 90.00

Refinement procedure

Resolution	38.910 - 2.770
R-factor	0.2323
Rwork	0.230
R-free	0.27910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.448
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.910	2.920
High resolution limit [Å]	2.770	2.770
Rmeas	0.102	1.004
Number of reflections	20022	2887
<I/σ(I)>	11.4	1.8
Completeness [%]	99.1
Redundancy	6.9
CC(1/2)	0.996	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	0.2 M ammonium sulfate, 0.1 M sodium acetate, 22% PEG4000