9C4W
Menin mutant - G331R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.714, 80.012, 124.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.329 - 1.398 |
| Rwork | 0.158 |
| R-free | 0.18120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.985 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.15) |
| Phasing software | MOLREP (11.0 / 22.07.2010/) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.330 | 1.470 |
| High resolution limit [Å] | 1.398 | 1.398 |
| Rmerge | 0.056 | 0.573 |
| Number of reflections | 96452 | 13763 |
| <I/σ(I)> | 16.7 | 2.5 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% (w/v) PEG-3,350 |
