9C4V

Menin mutant G331D in complex with MLL peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2023-04-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	49.082, 80.217, 124.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.830 - 1.470
Rwork	0.160
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.914
Data reduction software	HKL-2000 (722)
Data scaling software	HKL-2000 (722)
Phasing software	MOLREP (11.0 / 22.07.2010)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.469	1.469
Rmerge	0.103	0.923
Number of reflections	84613	4171
<I/σ(I)>	33.083	2.09
Completeness [%]	99.9
Redundancy	7.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.2 M lithium sulfate, 0.1 M HEPES, pH 7.5, 25% (w/v) PEG-3,350