9C38
Nir1 NirD domain dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 69.403, 69.403, 150.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 1.950 |
| R-factor | 0.1734 |
| Rwork | 0.172 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.082 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.870 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.279 | 2.671 |
| Rmeas | 0.285 | 2.730 |
| Rpim | 0.061 | 0.561 |
| Number of reflections | 27623 | 2366 |
| <I/σ(I)> | 6.28 | 1.23 |
| Completeness [%] | 95.0 | 87.63 |
| Redundancy | 22.5 | 23.4 |
| CC(1/2) | 0.993 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Tris, pH 7.5, 8.5% isoproponal, 21% glycerol, 12% PEG 4000 |
