9C37
Nir2 NirD domain dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 106.765, 106.765, 136.885 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.010 - 2.452 |
| R-factor | 0.2093 |
| Rwork | 0.206 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.444 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.461 | 2.696 |
| High resolution limit [Å] | 2.452 | 2.452 |
| Rmerge | 0.117 | 1.579 |
| Rmeas | 0.124 | 1.692 |
| Rpim | 0.041 | 0.595 |
| Number of reflections | 12644 | 633 |
| <I/σ(I)> | 10.6 | 1.4 |
| Completeness [%] | 95.4 | 76.9 |
| Redundancy | 9.1 | 7.7 |
| CC(1/2) | 0.997 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Lithium Sulfate, 8% (w/v) PEG 3350 |
