9C26
Cyan thermostable protein (CTP) 0.5 at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-07 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.481, 76.491, 81.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.260 - 2.000 |
| R-factor | 0.2204 |
| Rwork | 0.218 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.260 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.135 | |
| Rmeas | 0.151 | 0.483 |
| Rpim | 0.066 | 0.205 |
| Number of reflections | 17051 | 2458 |
| <I/σ(I)> | 8 | 3.1 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 5.1 | 5.4 |
| CC(1/2) | 0.991 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294.15 | 0.15 M Magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 6.5, 30% PEG 4000 |
