9C25
Cyan thermostable protein (CTP) 1.0 at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-17 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.332, 77.085, 81.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.600 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.917 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.630 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.089 | 1.215 |
| Rmeas | 0.092 | |
| Rpim | 0.026 | 0.708 |
| Number of reflections | 33405 | 1641 |
| <I/σ(I)> | 23.9 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22 | 6.1 |
| CC(1/2) | 1.000 | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294.15 | 0.15 M Magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 8.5, 27.5% PEG 4000 |
