9C22

Crystal structure of chimeric hemagglutinin cH11/1 in complex with broad protective antibody 3E1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	182.433, 194.500, 214.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.050 - 4.600
R-factor	0.2941
Rwork	0.293
R-free	0.32470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.748
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.050	4.680
High resolution limit [Å]	4.600	4.600
Number of reflections	42908	40617
<I/σ(I)>	4.4
Completeness [%]	99.8
Redundancy	14.4
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium phosphate dibasic, 20% w/v PEG3350