9C0Z

Clathrin terminal domain complexed with pitstop 2d

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-27
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	70.826, 74.486, 82.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.180 - 1.510
R-factor	0.16293
Rwork	0.162
R-free	0.18571
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.888
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.180	1.564
High resolution limit [Å]	1.509	1.509
Rmerge	0.072
Number of reflections	68885	6782
<I/σ(I)>	16.23
Completeness [%]	99.9
Redundancy	4.6
CC(1/2)		0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG 3350, 150 MM POTASSIUM ACETATE, 0.1 M TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K