9C0V

Crystal structure of chimeric hemagglutinin cH5/1 in complex with broad protective antibody 3E1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	I 21 3
Unit cell lengths	241.491, 241.491, 241.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.250 - 3.500
R-factor	0.2937
Rwork	0.292
R-free	0.32140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.494
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.280	3.560
High resolution limit [Å]	3.500	3.500
Number of reflections	29612	1459
<I/σ(I)>	20.3
Completeness [%]	100.0
Redundancy	41.6
CC(1/2)	0.990	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M sodium cacodylate, pH 6.5, 0.8 M sodium citrate