9C0U

Crystal structure of chimeric hemagglutinin cH5/1 in complex with broad protective antibody 31.b.09

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979
Spacegroup name	P 21 3
Unit cell lengths	162.079, 162.079, 162.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.870 - 3.590
R-factor	0.2967
Rwork	0.294
R-free	0.34350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.827
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	3.610
High resolution limit [Å]	3.590	3.590
Number of reflections	16876	15949
<I/σ(I)>	5.9
Completeness [%]	100.0
Redundancy	39.6
CC(1/2)	0.990	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris, pH 8.5, 20% w/v PEG1000