9BY5
Crystal Structure of RT-PhyR (ruthe_01174)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 113.910, 118.642, 121.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 1.990 |
| R-factor | 0.2101 |
| Rwork | 0.209 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.344 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.108 | 3.462 |
| Rmeas | 0.112 | 3.590 |
| Rpim | 0.030 | 0.944 |
| Number of reflections | 56844 | 2789 |
| <I/σ(I)> | 13.4 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 | 14.2 |
| CC(1/2) | 0.999 | 0.352 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.1M Tris.HCl pH 8.5, 16% PEG 10000 |
