9BXD

CRYSTAL STRUCTURE OF HIV-1 LM/HS CLADE A/E CRF01 GP120 CORE IN COMPLEX WITH HZ-IV-188

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-25
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.95369
Spacegroup name	P 21 21 21
Unit cell lengths	61.013, 65.837, 90.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.430 - 1.850
R-factor	0.2311
Rwork	0.230
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.997
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.065	0.636
Rpim	0.043	0.526
Number of reflections	29237	1143
<I/σ(I)>	23.6	0.8
Completeness [%]	92.4	74.2
Redundancy	2.8	1.8
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	10% PEG 3350 5% PEG 400 0.1 M HEPES pH 7.5