9BX5

Human Fab 8C1 in complex with OspCA peptide P4 (residues 141-144)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 21 2 21
Unit cell lengths	67.657, 69.389, 95.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.900 - 1.570
R-factor	0.143
Rwork	0.142
R-free	0.16970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.074
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.570	1.570
Number of reflections	63082	3046
<I/σ(I)>	51
Completeness [%]	99.8
Redundancy	12.3
CC(1/2)	0.999	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	200 mM sodium nitrate and 20% PEG 3,350