9BT5

The crystal structure of the HA1 domain of hemagglutinin from A/Shanghai/02/2013 (H7N9) bound to H7-235 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	240.603, 95.053, 99.265
Unit cell angles	90.00, 95.85, 90.00

Refinement procedure

Resolution	49.370 - 2.500
R-factor	0.1979
Rwork	0.195
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.113
Data scaling software	Aimless
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.096	0.862
Number of reflections	76766	11112
<I/σ(I)>	9.9	1.7
Completeness [%]	99.6	99.2
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 M sodium chloride, 8-10% PEG 6000