9BS5
Bacteroides ovatus GH97C Sus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-03-10 |
| Detector | MAR CCD 300 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.855, 116.528, 144.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.260 - 1.460 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.246 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.260 | 1.480 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.100 | |
| Number of reflections | 312566 | 10323 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.8 | |
| CC(1/2) | 0.990 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Molecular Dimensions Morpheus screen condition that included 100 mM NaHEPES/MOPS pH 7.5 (Thermo), 100 mM amino acids and 36% precipitant mix 4 |
