9BR5
IL1RAP-specific Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 31 |
| Unit cell lengths | 65.440, 65.440, 98.373 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.720 - 2.900 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.808 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.720 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmeas | 0.301 | |
| Number of reflections | 103107 | 1380 |
| <I/σ(I)> | 4.56 | 0.9 |
| Completeness [%] | 99.8 | 99.53 |
| Redundancy | 7.5 | 7.7 |
| CC(1/2) | 0.982 | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.17 M sodium acetate, 0.085 M Tris-Cl pH 8.5, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol |
