9BPS

Plasmodium falciparum apicoplast DNA polymerase mutant - K417M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	144.931, 144.931, 166.383
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.440 - 2.800
R-factor	0.2316
Rwork	0.231
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.720
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	48658	2415
<I/σ(I)>	14.5
Completeness [%]	100.0	100
Redundancy	11.3	9.9
CC(1/2)	0.977	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	Equal parts protein and precipitant solutions. Protein solution: 18 mg/ml protein, 20 mM Tris, pH 8.0, 500 mM NaCl. Precipitant solution: PEG-1000 30% (v/v), 100 sodium citrate tribasic dihydrate, pH 5.5, 500 mM NaCl