9BMK

GH5_4 endo-beta(1,3/1,4)-glucanase E331A from Segatella copri in complex with cellotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2024-03-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953
Spacegroup name	P 32 2 1
Unit cell lengths	107.550, 107.550, 232.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.800 - 2.210
R-factor	0.2221
Rwork	0.222
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.979
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PDB-REDO

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.840	2.289
High resolution limit [Å]	2.210	2.210
Rmerge	0.024	0.945
Number of reflections	149544	7730
<I/σ(I)>	14.14
Completeness [%]	99.5
Redundancy	1.9
CC(1/2)	1.000	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2 M ammonium sulfate, 0.1 M potassium sodium tartrate tetrahydrate