9BLA

KIR3DL1*086 in complex with HLA-A*24:02 presenting the NEF peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	P 1
Unit cell lengths	50.682, 61.790, 67.473
Unit cell angles	93.73, 100.68, 108.34

Refinement procedure

Resolution	46.960 - 3.000
R-factor	0.2024
Rwork	0.200
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5058)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.960	3.180
High resolution limit [Å]	3.000	3.000
Rmerge	0.063	0.355
Rpim	0.045
Number of reflections	14821	2422
<I/σ(I)>	5.8	2.8
Completeness [%]	97.9
Redundancy	2.8
CC(1/2)	0.996	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294	14% PEG 3350, 2% tacsimate pH 5.0, 0.1 M tri-sodium citrate pH 5.6