9BL9
KIR3DL1*114 in complex with HLA-A*24:02 presenting the NEF peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953659 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.532, 60.602, 64.635 |
| Unit cell angles | 93.96, 96.19, 105.50 |
Refinement procedure
| Resolution | 41.240 - 2.600 |
| R-factor | 0.2245 |
| Rwork | 0.224 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.485 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.360 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.472 |
| Number of reflections | 20269 | 2442 |
| <I/σ(I)> | 10.5 | 2 |
| Completeness [%] | 98.0 | 97.6 |
| Redundancy | 3.1 | |
| CC(1/2) | 0.996 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 14% PEG 3350, 2% tacsimate pH 5.0, 0.1 M tri-sodium citrate pH 5.6 |
