9BL8
MIT (L7C/A43C) Domain of Vps4p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 20.699, 42.939, 96.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.070 - 1.300 |
| R-factor | 0.1405 |
| Rwork | 0.136 |
| R-free | 0.18030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.847 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.070 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.133 | 0.481 |
| Rmeas | 0.150 | 0.555 |
| Rpim | 0.068 | 0.268 |
| Number of reflections | 19914 | 1397 |
| <I/σ(I)> | 7.95 | 1.72 |
| Completeness [%] | 90.0 | 92.59 |
| Redundancy | 3.9 | 3.33 |
| CC(1/2) | 0.983 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | Vps4 MIT Domain (10mg/ml) in 20mM Tris pH = 8, 100mM NaCl mixed in a 1:2 ratio with 0.2M Calcium Chloride dihydrate, 0.1M sodium acetate trihydrate pH=4.6, 20% 2-propanol |
