9BL1
Crystal structure of heme-binding protein from Populus trichocarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 77.306, 77.306, 92.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.680 - 1.800 |
| R-factor | 0.20881 |
| Rwork | 0.206 |
| R-free | 0.25470 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 1.030 |
| Rmeas | 0.061 | |
| Number of reflections | 25732 | 1726 |
| <I/σ(I)> | 10.9 | 2 |
| Completeness [%] | 96.7 | 79.5 |
| Redundancy | 20 | 8.3 |
| CC(1/2) | 1.000 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% PEG monomethyl ether 2000, 10 mM nickel chloride and 100 mM sodium acetate pH 5.0 |
