9BKI

Crystal structure of Rid family protein ACIAD3089 from Acinetobacter baylyi in C2 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	159.270, 77.380, 150.400
Unit cell angles	90.00, 97.42, 90.00

Refinement procedure

Resolution	43.530 - 2.380
R-factor	0.2013
Rwork	0.200
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.109
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.380	2.380
Rmerge	0.077	0.581
Number of reflections	70631	4701
<I/σ(I)>	14.85	3.88
Completeness [%]	99.4
Redundancy	6.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, 0.2M SODIUM SULFATE