9BKC
Crystal structure of Rid family protein PFL1385 from Pseudomonas fluorescens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 43 |
Unit cell lengths | 84.010, 84.010, 123.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.570 - 2.490 |
R-factor | 0.2002 |
Rwork | 0.197 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.132 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.059 | 0.541 |
Number of reflections | 29451 | 1987 |
<I/σ(I)> | 23.36 | 4.42 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.46 | 11.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 8.5, 1.5 M Ammonium sulfate |