9BK9

Crystal structure of Rid family protein PA0814 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	46.970, 84.647, 85.789
Unit cell angles	90.00, 90.71, 90.00

Refinement procedure

Resolution	37.960 - 1.780
R-factor	0.1885
Rwork	0.188
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.063	0.941
Number of reflections	62539	3070
<I/σ(I)>	35.13	3.9
Completeness [%]	97.4
Redundancy	7.7
CC(1/2)		0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6,30% w/v Polyethylene glycol 4,000