9BK6

Structure of B10_CYTX binder-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 21 21 21
Unit cell lengths	34.564, 63.663, 77.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.170 - 2.000
R-factor	0.2514
Rwork	0.250
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.438
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_5288)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.170	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.599
Rpim	0.041	0.281
Number of reflections	12102	864
<I/σ(I)>	10.4	2.6
Completeness [%]	99.7	99.7
Redundancy	6.4	6.2
CC(1/2)	0.994	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M MES pH 6, 0.01 M Zinc chloride, 20% (w/v) PEG 6000 and 10 % (v/v) Ethylene glycol