9BK1
Crystal structure of Endoribonuclease L-PSP family protein PSPTO0102 Sulfur SAD phased
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-13 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 66.684, 66.684, 66.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.150 - 1.570 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.20540 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.707 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.029 | 0.073 |
| Rmeas | 0.029 | 0.099 |
| Rpim | 0.005 | 0.066 |
| Number of reflections | 13020 | 153 |
| <I/σ(I)> | 118.35 | 7.45 |
| Completeness [%] | 94.1 | |
| Redundancy | 28.5 | |
| CC(1/2) | 0.984 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Magnesium formate dihydrate, 15% w/v Polyethylene glycol 3,350 |
