9BJX

GH5_4 endo-beta(1,3/1,4)-glucanase from Segatella copri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 1 21 1
Unit cell lengths	97.008, 51.560, 157.057
Unit cell angles	90.00, 101.50, 90.00

Refinement procedure

Resolution	38.950 - 1.600
R-factor	0.178
Rwork	0.177
R-free	0.20050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.838
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.950	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.025	0.310
Number of reflections	197039	19223
<I/σ(I)>	14.59	2.47
Completeness [%]	97.8	96.03
Redundancy	2
CC(1/2)	1.000	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M LiCl, 19% (w/v) Polyethylene glycol 3,350