9BJW

Crystal structure of RidA family protein PA5083 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	75.480, 75.480, 140.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.740 - 1.540
R-factor	0.1995
Rwork	0.198
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.856
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.061	0.465
Rmeas	0.063
Rpim	0.015
Number of reflections	22725	1132
<I/σ(I)>	68.87	4.31
Completeness [%]	99.9	100
Redundancy	17.2
CC(1/2)	0.997	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate