9BJW
Crystal structure of RidA family protein PA5083 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 75.480, 75.480, 140.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.740 - 1.540 |
R-factor | 0.1995 |
Rwork | 0.198 |
R-free | 0.21280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.856 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.061 | 0.465 |
Rmeas | 0.063 | |
Rpim | 0.015 | |
Number of reflections | 22725 | 1132 |
<I/σ(I)> | 68.87 | 4.31 |
Completeness [%] | 99.9 | 100 |
Redundancy | 17.2 | |
CC(1/2) | 0.997 | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate |