9BJL
Crystal structure of Influenza D virus Nucleoprotein (Oklahoma)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-04-13 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 |
Unit cell lengths | 74.461, 84.501, 88.728 |
Unit cell angles | 102.34, 97.37, 97.14 |
Refinement procedure
Resolution | 85.570 - 2.430 |
R-factor | 0.2051 |
Rwork | 0.203 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.761 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.570 | 2.490 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.075 | 0.630 |
Rmeas | 0.095 | 0.802 |
Rpim | 0.058 | 0.489 |
Total number of observations | 169069 | 13744 |
Number of reflections | 71662 | 5626 |
<I/σ(I)> | 8.2 | 1.5 |
Completeness [%] | 91.9 | |
Redundancy | 2.4 | 2.4 |
CC(1/2) | 0.997 | 0.591 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 2.4M Malonate, pH 4.0, InvdC.18714.a.PQ11.PS38729 at 40 mg/mL. plate Liu-S-115 C2, Puck: PSL-0806, Cryo: 3.4M Malonate, pH 7.0 |