9BJL

Crystal structure of Influenza D virus Nucleoprotein (Oklahoma)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-13
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 1
Unit cell lengths	74.461, 84.501, 88.728
Unit cell angles	102.34, 97.37, 97.14

Refinement procedure

Resolution	85.570 - 2.430
R-factor	0.2051
Rwork	0.203
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.761
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.570	2.490
High resolution limit [Å]	2.430	2.430
Rmerge	0.075	0.630
Rmeas	0.095	0.802
Rpim	0.058	0.489
Total number of observations	169069	13744
Number of reflections	71662	5626
<I/σ(I)>	8.2	1.5
Completeness [%]	91.9
Redundancy	2.4	2.4
CC(1/2)	0.997	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	2.4M Malonate, pH 4.0, InvdC.18714.a.PQ11.PS38729 at 40 mg/mL. plate Liu-S-115 C2, Puck: PSL-0806, Cryo: 3.4M Malonate, pH 7.0