9BJH
Crystal structure of neutralizing human monoclonal antibody 75C8 in complex with AMA1 DI-DII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.868, 71.620, 132.927 |
| Unit cell angles | 94.11, 99.19, 112.39 |
Refinement procedure
| Resolution | 19.870 - 2.800 |
| R-factor | 0.2128 |
| Rwork | 0.211 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.473 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.20.1-4487) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.870 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.112 | 0.482 |
| Rmeas | 0.159 | 0.681 |
| Rpim | 0.112 | 0.482 |
| Number of reflections | 41930 | 3285 |
| <I/σ(I)> | 3.52 | 0.69 |
| Completeness [%] | 86.6 | 74.25 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.976 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291.15 | 50% (v/v) PEG 400, 0.2M sodium chloride, 0.1M CHES |
