9BJ6
Crystal structure of the HEPN family member AbiV, an RNase in a two-component antiphage system in Lactococcus lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.173, 144.955, 55.563 |
| Unit cell angles | 90.00, 95.53, 90.00 |
Refinement procedure
| Resolution | 72.480 - 2.920 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.777 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 144.960 | 3.080 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.134 | 0.948 |
| Rmeas | 0.156 | 1.099 |
| Rpim | 0.080 | 0.555 |
| Total number of observations | 69090 | 10239 |
| Number of reflections | 18073 | 2649 |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 99.2 | |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.995 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 1.0M Lithium chloride, 0.1M Sodium acetate, 30% PEG 6000 |
