9BJ0
Crystal structure of the periplasmic domain of IgaA from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97911 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.421, 94.179, 160.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.360 - 2.640 |
R-factor | 0.2182 |
Rwork | 0.216 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.228 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.740 |
High resolution limit [Å] | 2.640 | 2.650 |
Rmeas | 0.083 | |
Number of reflections | 20383 | 1993 |
<I/σ(I)> | 29.5 | |
Completeness [%] | 99.6 | |
Redundancy | 6 | |
CC(1/2) | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | 20% PEG3350, 0.2 M potassium citrate |