9BIY
Crystal structure of the periplasmic domain of IgaA from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-04-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.382, 38.266, 120.068 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 39.870 - 1.800 |
| R-factor | 0.2418 |
| Rwork | 0.240 |
| R-free | 0.27570 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.934 |
| Data reduction software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.870 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.063 | 1.882 |
| Number of reflections | 30909 | 5234 |
| <I/σ(I)> | 9.3 | 2.1 |
| Completeness [%] | 93.1 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350, 0.2 M potassium citrate |
